To perform automated sequential imaging, we use a Nikon microscope coupled to a Yokogawa CSU-W1 confocal scanner. It includes a 405 nm, 488 nm, 561 nm, 638 nm and a 690 nm laser for barcode demultiplexing in four channels.
Microfluidics
Elveflow sequential injection system
This custom-built microfluidics system comprises several distribution valves and enables the automated delivery of up to 47 solutions into a microfluidics imaging chamber.
Aria Fluigent sequential injection system
This system can be used for the automated delivery of up to 10 different solutions into a microfluidic imaging chamber. For automated sequential imaging experiments, the device can be synchronised with a microscope using a TTL signal.
Image Analysis
For processing and analysing imaging datasets at scale, we can offer remote access to high powered virtual machines via the EMBL 3D cloud infrastructure.