To perform automated sequential imaging, we use a Nikon microscope coupled to a Yokogawa CSU-W1 confocal scanner. It includes a 405 nm, 488 nm, 561 nm, 638 nm and a 690 nm laser for barcode demultiplexing in four channels.
Microfluidics
Elveflow sequential injection system
This custom-built microfluidics system comprises several distribution valves and enables the automated delivery of up to 47 solutions into a microfluidics imaging chamber.
Aria Fluigent sequential injection system
This system can be used for the automated delivery of up to 10 different solutions into a microfluidic imaging chamber. For automated sequential imaging experiments, the device can be synchronised with a microscope using a TTL signal.
Image Analysis
Schematic of data analysis workflow
For processing and analysing imaging datasets at scale, we can offer remote access to high powered virtual machines via the EMBL 3D cloud infrastructure.