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Genome Technology Development

Custom multi-omics tech development

Instrumentation

Microscopy

confocal spinning disk

Nikon Yokogawa spinning disk confocal

To perform automated sequential imaging, we use a Nikon microscope coupled to a Yokogawa CSU-W1 confocal scanner. It includes a 405 nm, 488 nm, 561 nm, 638 nm and a 690 nm laser for barcode demultiplexing in four channels.

Microfluidics

Elveflow sequential injection

Elveflow sequential injection system

This custom-built microfluidics system comprises several distribution valves and enables the automated delivery of up to 47 solutions into a microfluidics imaging chamber.

Aria Fluigent sequential injection

Aria Fluigent sequential injection system

This system can be used for the automated delivery of up to 10 different solutions into a microfluidic imaging chamber. For automated sequential imaging experiments, the device can be synchronised with a microscope using a TTL signal.

Image Analysis

Schematic of data analysis workflow

For processing and analysing imaging datasets at scale, we can offer remote access to high powered virtual machines via the EMBL 3D cloud infrastructure.

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