Structural basis of thiamine transport and drug recognition by SLC19A3.

Gabriel F, Spriestersbach L, Fuhrmann A, Jungnickel KEJ, Mostafavi S, Pardon E, Steyaert J, Löw C

Nature communications, 2024

doi:10.1038/s41467-024-52872-8.

MFSD1 with its accessory subunit GLMP functions as a general dipeptide uniporter in lysosomes.

Jungnickel KEJ, Guelle O, Iguchi M, Dong W, Kotov V, Gabriel F, Debacker C, Dairou J, McCort-Tranchepain I, Laqtom NN, Chan SH, Ejima A, Sato K, Massa López D, Saftig P, Mehdipour AR, Abu-Remaileh M, Gasnier B, Löw C, Damme M

Nature cell biology, 2024

doi:10.1038/s41556-024-01436-5.

Transient co-expression of membrane protein complexes in mammalian cells.

Custódio TF, Guédez G, Löw C

Methods in molecular biology (Clifton, N.J.), 2024

doi:10.1007/978-1-0716-3878-1_2.

Form factor determination of biological molecules with X-ray free electron laser small-angle scattering (XFEL-SAS).

Blanchet CE, Round A, Mertens HDT, Ayyer K, Graewert M, Awel S, Franke D, Dörner K, Bajt S, Bean R, Custódio TF, de Wijn R, Juncheng E, Henkel A, Gruzinov A, Jeffries CM, Kim Y, Kirkwood H, Kloos M, Knoška J, Koliyadu J, Letrun R, Löw C, Makroczyova J, Mall A, Meijers R, Pena Murillo GE, Oberthür D, Round E, Seuring C, Sikorski M, Vagovic P, Valerio J, Wollweber T, Zhuang Y, Schulz J, Haas H, Chapman HN, Mancuso AP, Svergun D

Communications biology, 2023

doi:10.1038/s42003-023-05416-7.

Plastid-localized xanthorhodopsin increases diatom biomass and ecosystem productivity in iron-limited surface oceans.

Strauss J, Deng L, Gao S, Toseland A, Bachy C, Zhang C, Kirkham A, Hopes A, Utting R, Joest EF, Tagliabue A, Löw C, Worden AZ, Nagel G, Mock T

Nature microbiology, 2023

doi:10.1038/s41564-023-01498-5.

Molecular basis of TASL recruitment by the peptide/histidine transporter 1, PHT1.

Custódio TF, Killer M, Yu D, Puente V, Teufel DP, Pautsch A, Schnapp G, Grundl M, Kosinski J, Löw C

Nature communications, 2023

doi:10.1038/s41467-023-41420-5.

Sybodies as novel bioreceptors toward field-effect transistor-based detection of SARS-CoV-2 antigens.

Zhang C, Parichenko A, Choi W, Shin S, Panes-Ruiz LA, Belyaev D, Custódio TF, Löw C, Lee JS, Ibarlucea B, Cuniberti G

ACS applied materials & interfaces, 2023

doi:10.1021/acsami.3c06073.

Plasticity of the binding pocket in peptide transporters underpins promiscuous substrate recognition.

Kotov V, Killer M, Jungnickel KEJ, Lei J, Finocchio G, Steinke J, Bartels K, Strauss J, Dupeux F, Humm AS, Cornaciu I, Márquez JA, Pardon E, Steyaert J, Löw C

Cell reports, 2023

doi:10.1016/j.celrep.2023.112831.

In vitro and ex vivo proteomics of Mycobacterium marinum biofilms and the development of biofilm-binding synthetic nanobodies.

Hammarén MM, Luukinen H, Sillanpää A, Remans K, Lapouge K, Custódio T, Löw C, Myllymäki H, Montonen T, Seeger M, Robertson J, Nyman TA, Savijoki K, Parikka M

mSystems, 2023

doi:10.1128/msystems.01073-22.

X-ray structure and function of fibronectin domains two and three of the neural cell adhesion molecule L1.

Guédez G, Loers G, Jeffries CM, Kozak S, Meijers R, Svergun DI, Schachner M, Löw C

FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2023

doi:10.1096/fj.202201511R.

Adsorption and Inactivation of SARS-CoV-2 on the Surface of Anatase TiO2(101).

Kohantorabi M, Wagstaffe M, Creutzburg M, Ugolotti A, Kulkarni S, Jeromin A, Krekeler T, Feuerherd M, Herrmann A, Ebert G, Protzer U, Guédez G, Löw C, Thuenauer R, Schlueter C, Gloskovskii A, Keller TF, Di Valentin C, Stierle A, Noei H

ACS applied materials & interfaces, 2023

doi:10.1021/acsami.2c22078.

Functional inactivation of Plasmodium falciparum glycogen synthase kinase GSK3 modulates erythrocyte invasion and blocks gametocyte maturation.

Alder A, Wilcke L, Pietsch E, von Thien H, Pazicky S, Löw C, Mesen-Ramirez P, Bachmann A, Burda PC, Kunick C, Sondermann H, Wilson D, Gilberger TW

The Journal of biological chemistry, 2022

doi:10.1016/j.jbc.2022.102360.

Cryo-EM structure of an atypical Proton-coupled Peptide Transporter: Di- and tripeptide Permease C.

Killer M, Finocchio G, Mertens HDT, Svergun DI, Pardon E, Steyaert J, Löw C

Frontiers in molecular biosciences, 2022

doi:10.3389/fmolb.2022.917725.

PMRT1, a Plasmodium-specific parasite plasma membrane transporter, is essential for asexual and sexual blood stage development.

Wichers JS, Mesén-Ramírez P, Fuchs G, Yu-Strzelczyk J, Stäcker J, von Thien H, Alder A, Henshall I, Liffner B, Nagel G, Löw C, Wilson D, Spielmann T, Gao S, Gilberger TW, Bachmann A, Strauss J

mBio, 2022

doi:10.1128/mbio.00623-22.

Impact of distant peptide substrate residues on enzymatic activity of SlyD.

Pazicky S, Werle AA, Lei J, Löw C, Weininger U

Cellular and molecular life sciences : CMLS, 2022

doi:10.1007/s00018-022-04179-4.

N-terminal phosphorylation regulates the activity of Glycogen Synthase Kinase 3 from Plasmodium falciparum.

Pazicky S, Alder A, Mertens H, Svergun DI, Gilberger T, Löw C

The Biochemical journal, 2022

doi:10.1042/BCJ20210829.

Completing the family of human EH domains: solution structure of the internal EH domain of γ-synergin.

Kovermann M, Weininger U, Löw C

Protein science : a publication of the Protein Society, 2021

doi:10.1002/pro.4269.

Structural snapshots of human PepT1 and PepT2 reveal mechanistic insights into substrate and drug transport across epithelial membranes.

Killer M, Wald J, Pieprzyk J, Marlovits TC, Löw C

Science advances, 2021

doi:10.1126/sciadv.abk3259.

Deamidation drives molecular aging of the SARS-CoV-2 spike protein receptor-binding motif.

Lorenzo R, Defelipe LA, Aliperti L, Niebling S, Custódio TF, Löw C, Schwarz JJ, Remans K, Craig PO, Otero LH, Klinke S, García-Alai M, Sánchez IE, Alonso LG

The Journal of biological chemistry, 2021

doi:10.1016/j.jbc.2021.101175.

Molecular basis of mRNA transport by a kinesin-1-atypical tropomyosin complex.

Dimitrova-Paternoga L, Jagtap PKA, Cyrklaff A, Vaishali, Lapouge K, Sehr P, Perez K, Heber S, Löw C, Hennig J, Ephrussi A

Genes & development, 2021

doi:10.1101/gad.348443.121.

Single-molecule FRET of membrane transport proteins.

Bartels K, Lasitza-Male T, Hofmann H, Löw C

Chembiochem : a European journal of chemical biology, 2021

doi:10.1002/cbic.202100106.

Identification of novel inner membrane complex and apical annuli proteins of the malaria parasite Plasmodium falciparum.

Wichers JS, Wunderlich J, Heincke D, Pazicky S, Strauss J, Schmitt M, Kimmel J, Wilcke L, Scharf S, von Thien H, Burda PC, Spielmann T, Löw C, Filarsky M, Bachmann A, Gilberger TW

Cellular microbiology, 2021

doi:10.1111/cmi.13341.

Selection, biophysical and structural analysis of synthetic nanobodies that effectively neutralize SARS-CoV-2.

Custódio TF, Das H, Sheward DJ, Hanke L, Pazicky S, Pieprzyk J, Sorgenfrei M, Schroer MA, Gruzinov AY, Jeffries CM, Graewert MA, Svergun DI, Dobrev N, Remans K, Seeger MA, McInerney GM, Murrell B, Hällberg BM, Löw C

Nature communications, 2020

doi:10.1038/s41467-020-19204-y.

In-depth interrogation of protein thermal unfolding data with MoltenProt.

Kotov V, Mlynek G, Vesper O, Pletzer M, Wald J, Teixeira-Duarte CM, Celia H, Garcia-Alai M, Nussberger S, Buchanan SK, Morais-Cabral JH, Loew C, Djinovic-Carugo K, Marlovits TC

Protein science : a publication of the Protein Society, 2020

doi:10.1002/pro.3986.

Structural role of essential light chains in the apicomplexan glideosome.

Pazicky S, Dhamotharan K, Kaszuba K, Mertens HDT, Gilberger T, Svergun D, Kosinski J, Weininger U, Löw C

Communications biology, 2020

doi:10.1038/s42003-020-01283-8.

Characterization of the complex of the lysosomal membrane transporter MFSD1 and its accessory subunit GLMP.

López DM, Kählau L, Jungnickel KEJ, Löw C, Damme M

FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2020

doi:10.1096/fj.202000912RR.

Membrane chemistry tunes the structure of a peptide transporter.

Lasitza-Male T, Bartels K, Jungwirth J, Wiggers F, Rosenblum G, Hofmann H, Löw C

Angewandte Chemie (International ed. in English), 2020

doi:10.1002/anie.202008226.

Structural insights into PfARO and characterization of its interaction with PfAIP.

Geiger M, Brown C, Wichers JS, Strauss J, Lill A, Thuenauer R, Liffner B, Wilcke L, Lemcke S, Heincke D, Pazicky S, Bachmann A, Löw C, Wilson D, Filarsky M, Zhang K, Burda PC, Gilberger TW, Junop M

Journal of molecular biology, 2019

doi:10.1016/j.jmb.2019.12.024.

Dissecting the gene expression, localization, membrane topology, and function of the Plasmodium falciparum STEVOR protein family.

Wichers JS, Scholz JAM, Strauss J, Witt S, Lill A, Ehnold LI, Neupert N, Liffner B, Lühken R, Petter M, Lorenzen S, Wilson DW, Löw C, Lavazec C, Bruchhaus I, Gilberger TW, Tannich E, Bachmann A

mBio, 2019

doi:10.1128/mBio.01500-19.

High-throughput stability screening for detergent-solubilized membrane proteins.

Kotov V, Bartels K, Veith K, Josts I, Subhramanyam UKT, Günther C, Labahn J, Marlovits TC, Moraes I, Tidow H, Löw C, Garcia-Alai MM

Scientific reports, 2019

doi:10.1038/s41598-019-46686-8.

Structure of prototypic peptide transporter DtpA from E. coli in complex with valganciclovir provides insights into drug binding of human PepT1.

Ural-Blimke Y, Flayhan A, Strauss J, Rantos V, Bartels K, Nielsen R, Pardon E, Steyaert J, Kosinski J, Quistgaard EM, Low C

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2019

doi:10.1021/jacs.8b11343.

Probing the architecture of a multi-PDZ domain protein: structure of PDZK1 in solution.

Hajizadeh N, Pieprzyk J, Skopintsev P, Flayhan A, Svergun DI, Loew C

2018

doi:10.1016/j.str.2018.07.016.

Tripeptide binding in a Proton-Dependent Oligopeptide Transporter.

Martinez Molledo M, Quistgaard EM, Löw C

FEBS letters, 2018

doi:10.1002/1873-3468.13246.

Transient Expression of Recombinant Membrane-eGFP Fusion Proteins in HEK293 Cells.

Pieprzyk J, Pazicky S, Löw C

Methods in molecular biology (Clifton, N.J.), 2018

doi:10.1007/978-1-4939-8730-6_2.

Multispecific Substrate Recognition in a Proton-Dependent Oligopeptide Transporter

Martinez Molledo M, Quistgaard EM, Flayhan A, Pieprzyk J, Löw C

2018

doi:10.1016/j.str.2018.01.005.

Saposin Lipid Nanoparticles: A Highly Versatile and Modular Tool for Membrane Protein Research.

Flayhan A, Mertens HDT, Ural-Blimke Y, Martinez Molledo M, Svergun DI, Löw C

Structure (London, England : 1993), 2018

doi:10.1016/j.str.2018.01.007.

Lipid-like Peptides can Stabilize Integral Membrane Proteins for Biophysical and Structural Studies.

Veith K, Martinez Molledo M, Almeida Hernandez Y, Josts I, Nitsche J, Löw C, Tidow H

Chembiochem : a European journal of chemical biology, 2017

doi:10.1002/cbic.201700235.

Structure determination of a major facilitator peptide transporter: Inward facing PepTSt from Streptococcus thermophilus crystallized in space group P3121.

Quistgaard EM, Martinez Molledo M, Löw C

PloS one, 2017

doi:10.1371/journal.pone.0173126.

Molecular insights into substrate recognition and catalytic mechanism of the chaperone and FKBP peptidyl-prolyl isomerase SlyD

Quistgaard EM, Weininger U, Ural-Blimke Y, Modig K, Nordlund P, Akke M, Low C

BMC BIOLOGY, 2016

doi:10.1186/s12915-016-0300-3.

A saposin-lipoprotein nanoparticle system for membrane proteins.

Frauenfeld J, Löving R, Armache JP, Sonnen AF, Guettou F, Moberg P, Zhu L, Jegerschöld C, Flayhan A, Briggs JA, Garoff H, Löw C, Cheng Y, Nordlund P

Nature methods, 2016

doi:10.1038/nmeth.3801.

Understanding transport by the major facilitator superfamily (MFS): structures pave the way.

Quistgaard EM, Löw C, Guettou F, Nordlund P

Nature reviews. Molecular cell biology, 2016

doi:10.1038/nrm.2015.25.

Selectivity mechanism of a bacterial homolog of the human drug-peptide transporters PepT1 and PepT2.

Guettou F, Quistgaard EM, Raba M, Moberg P, Löw C, Nordlund P

Nature structural & molecular biology, 2014

doi:10.1038/nsmb.2860.

Structural and Biochemical Characterization of Human PR70 in Isolation and in Complex with the Scaffolding Subunit of Protein Phosphatase 2A

Dovega R, Tsutakawa S, Quistgaard EM, Anandapadamanaban M, Löw C, Nordlund P

PloS one, 2014

doi:10.1371/journal.pone.0101846.

Structural basis for PTPA interaction with the invariant C-terminal tail of PP2A

Löw C, Quistgaard EM, Kovermann M, Anandapadamanaban M, Balbach J, Nordlund P

Biological chemistry, 2014

doi:10.1515/hsz-2014-0106.

Metal-mediated crystallization of the xylose transporter XylE from Escherichia coli in three different crystal forms

Quistgaard EM, Löw C, Moberg P, Nordlund P

Journal of structural biology, 2013

doi:10.1016/j.jsb.2013.09.009.

Structural insights into substrate recognition in proton-dependent oligopeptide transporters

Guettou F, Quistgaard EM, Trésaugues L, Moberg P, Jegerschöld C, Zhu L, Jong AJ, Nordlund P, Löw C

EMBO reports, 2013

doi:10.1038/embor.2013.107.

Structural and biophysical characterization of the cytoplasmic domains of human BAP29 and BAP31

Quistgaard EM, Löw C, Moberg P, Guettou F, Maddi K, Nordlund P

PloS one, 2013

doi:10.1371/journal.pone.0071111.

Slow aromatic ring flips detected despite near-degenerate NMR frequencies of the exchanging nuclei

Weininger U, Respondek M, Löw C, Akke M

The journal of physical chemistry. B, 2013

doi:10.1021/jp4058065.

High-throughput analytical gel filtration screening of integral membrane proteins for structural studies

Löw C, Moberg P, Quistgaard EM, Hedrén M, Guettou F, Frauenfeld J, Haneskog L, Nordlund P

Biochimica et biophysica acta, 2013

doi:10.1016/j.bbagen.2013.02.001.

Structural basis for substrate transport in the GLUT-homology family of monosaccharide transporters

Quistgaard EM, Löw C, Moberg P, Trésaugues L, Nordlund P

Nature structural & molecular biology, 2013

doi:10.1038/nsmb.2569.

Rapid screening of integral membrane proteins – detergents suitable for structure determination

Guettou F, Löw C, Quistgard E, Hedrén M, Frauenfeld J, Nordlund P, Haneskog L, Moberg P

2013

doi:.

Nanobody mediated crystallization of an archeal mechanosensitive channel

Löw C, Yau YH, Pardon E, Jegerschöld C, Wåhlin L, Quistgaard EM, Moberg P, Geifman-Shochat S, Steyaert J, Nordlund P

PloS one, 2013

doi:10.1371/journal.pone.0077984.

High-resolution insights into binding of unfolded polypeptides by the PPIase chaperone SlpA

Quistgaard EM, Nordlund P, Löw C

FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2012

doi:10.1096/fj.12-208397.

Metallochaperone SlyD

Löw C, Stubbs MT, Haupt C, Balbach J

2012

doi:10.1002/9781119951438.eibc2061.

Optimisation of over-expression in E. coli and biophysical characterisation of human membrane protein synaptogyrin 1

Löw C, Jegerschöld C, Kovermann M, Moberg P, Nordlund P

PloS one, 2012

doi:10.1371/journal.pone.0038244.

Escherichia coli peptide binding protein OppA has a preference for positively charged peptides

Klepsch MM, Kovermann M, Löw C, Balbach J, Permentier HP, Fusetti F, de Gier JW, Slotboom DJ, Berntsson RP

Journal of molecular biology, 2011

doi:10.1016/j.jmb.2011.09.043.

Conformational plasticity and dynamics in the generic protein folding catalyst SlyD unraveled by single-molecule FRET

Kahra D, Kovermann M, Löw C, Hirschfeld V, Haupt C, Balbach J, Hübner CG

Journal of molecular biology, 2011

doi:10.1016/j.jmb.2011.05.002.

NMR relaxation unravels interdomain crosstalk of the two domain prolyl isomerase and chaperone SlyD

Kovermann M, Zierold R, Haupt C, Löw C, Balbach J

Biochimica et biophysica acta, 2011

doi:10.1016/j.bbapap.2011.03.016.

Stability of membrane proteins analyzed by gel filtration

Moberg P, Burstedt M, Nordlund P, Haneskog L, Löw C

2011

doi:.

Rapid buffer scouting and quality control of integral membrane proteins

Löw C, Hedrén M, Nordlund P, Haneskog L, Moberg P

2011

doi:.

Structural studies of the human tetraspan vesicle membrane proteins

Loew C, Nordlund P

FEBS JOURNAL, 2010

doi:.

Crystal structure determination and functional characterization of the metallochaperone SlyD from Thermus thermophilus

Löw C, Neumann P, Tidow H, Weininger U, Haupt C, Friedrich-Epler B, Scholz C, Stubbs MT, Balbach J

Journal of molecular biology, 2010

doi:10.1016/j.jmb.2010.03.014.

Energy Landscapes of Protein Folding: From Structure to Function

Löw C

2009

doi:.

Structure-based stability analysis of an extremely stable dimeric DNA binding protein from Sulfolobus islandicus

Weininger U, Zeeb M, Neumann P, Löw C, Stubbs MT, Lipps G, Balbach J

Biochemistry, 2009

doi:10.1021/bi900760n.

The folding pathway of onconase is directed by a conserved intermediate

Schulenburg C, Löw C, Weininger U, Mrestani-Klaus C, Hofmann H, Balbach J, Ulbrich-Hofmann R, Arnold U

Biochemistry, 2009

doi:10.1021/bi900596j.

Fast amide proton exchange reveals close relation between native-state dynamics and unfolding kinetics

Hofmann H, Weininger U, Löw C, Golbik RP, Balbach J, Ulbrich-Hofmann R

Journal of the American Chemical Society, 2009

doi:10.1021/ja8048942.

Conformational switch upon phosphorylation: human CDK inhibitor p19INK4d between the native and partially folded state

Löw C, Homeyer N, Weininger U, Sticht H, Balbach J

ACS chemical biology, 2009

doi:10.1021/cb800219m.

Structure and dynamics of helix-0 of the N-BAR domain in lipid micelles and bilayers

Löw C, Weininger U, Lee H, Schweimer K, Neundorf I, Beck-Sickinger AG, Pastor RW, Balbach J

Biophysical journal, 2008

doi:10.1529/biophysj.108.134155.

Structural insights into an equilibrium folding intermediate of an archaeal ankyrin repeat protein

Löw C, Weininger U, Neumann P, Klepsch M, Lilie H, Stubbs MT, Balbach J

Proceedings of the National Academy of Sciences of the United States of America, 2008

doi:10.1073/pnas.0710657105.

Identification of three phases in Onconase refolding

Schulenburg C, Martinez-Senac MM, Löw C, Golbik R, Ulbrich-Hofmann R, Arnold U

The FEBS journal, 2007

doi:10.1111/j.1742-4658.2007.06106.x.

Folding mechanism of an ankyrin repeat protein: scaffold and active site formation of human CDK inhibitor p19(INK4d)

Löw C, Weininger U, Zeeb M, Zhang W, Laue ED, Schmid FX, Balbach J

Journal of molecular biology, 2007

doi:10.1016/j.jmb.2007.07.063.

Recognition of T-rich single-stranded DNA by the cold shock protein Bs-CspB in solution

Zeeb M, Max KE, Weininger U, Löw C, Sticht H, Balbach J

Nucleic acids research, 2006

doi:10.1093/nar/gkl376.