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Mesoscopic Imaging Facility

Viventis LS1

24 hours time lapse of mouse embryonic organoid development (maximum intensity projection). Magenta: ubiquitous nuclear label, green: T-Bra gene expression. Field of view: ~ 600 μm. Images acquired by Kerim Anlaş.

Available Objective Lenses

BrandTypeMag.
(Range)
NAImmersionWD [mm]F.o.V. [μm]Usage
Nikon25x
(18.75 – 37.5)
1.1Water400 x 400 – 798 x 798Detection
NikonPlan Fluor10x
(n.a.)
0.3Air16n.a.Illumination
NikonPlan Fluor10x
(n.a.)
0.3Air16n.a.Illumination

Available lasers

Wavelength [nm]
405
488
561
638

Available filters

Type Fluorophore(s)
Semrock Chroma ZET405/488/561/640mDapi/BFP, EGFP, RFP/dsRed, Alexa 647
Semrock Chroma FF03-525/50-25GFP, EGFP
Semrock Em01-R488/568-25EGFP, RFP/dsRed

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