OPTiSPIM2 – Mesoscopic Imaging Facility

Available objective lenses

Brand	Type	Mag.	NA	Immer- sion (ref. ind.)	WD [mm]	Qty.	Parfocal length [mm]	f_TL [mm]	F.o.V. [mm]	Illum. lens
Mitutoyo	M Plan Apo	1	0.025	Air (1.0)	11.0	1	95	200	16.6 x 14.0	–
Mitutoyo	M Plan Apo	2	0.055	Air (1.0)	34.0	2	95	200	8.32 x 7.02	–
Mitutoyo	M Plan Apo	5	0.14	Air (1.0)	34.0	3	95	200	3.328 x 2.808	#2
Mitutoyo	M Plan Apo	7.5	0.21	Air (1.0)	35.0	2	95	200	2.219 x 1.872	#2
Mitutoyo	M Plan Apo	10	0.28	Air (1.0)	33.5	2	95	200	1.664 x 1.404	#2
Mitutoyo	M Plan Apo	10	0.42	Air (1.0)	15.0	1	95	200	1.664 x 1.404	#2
Mitutoyo	M Plan Apo SL	20	0.28	Air (1.0)	30.5	1	95	200	0.832 x 0.702	#3
Zeiss	Plan- Apochromat	20*	1.0	Scale (1.38)	5.6	1	85	165*	0.686 x 0.579	#3

f_TL = tube lens focal length for designed magnification

* The OPTiSPIM2 has f_TL = 200 mm, so the effective magnification of the Zeiss lens is (20x)*(200 mm/165 mm) = 24.24x. This is taken into account for the F.o.V. calculations.
F.o.V. = field of view, with the Andor Neo sCMOS camera (2560 x 2160 pixels, 6.5 μm pitch)
Illum. lens = “optimal” lens for SPIM illumination

Available lasers

Type	Wavelength [nm]	Power [mW]
diode	405	100
diode	488	50
DPSS	561	50

Available filters, SPIM filter wheel

Type	Fluorphore(s)	Position
Opaque
Clear
447 BS 60	Blocks 405nm, CFP
525 BP 50	GFP, YFP, Alexa 488
593 BP 46	dsRed, Alexa 543, Cy3
685 BP 80	draq5, Alexa 633
LP 405
RS 488 LP
635 LP

Position = current position in filter wheel