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Mesoscopic Imaging Facility

Luxendo LCS SPIM

The LCS SPIM is a compact light-sheet microscope designed to scan large optically cleared samples. Samples can be fit in three cuvette sizes -from [20 mm × 20 mm × 20 mm] up to [100 mm x 30 mm x 30 mm] (l × w × h)- allowing imaging from organs to organisms.

LCS SPIM is an inverted setup with dual-side illumination and an extended field of view (FOV). It has the option of de-striping and also a TAG lens to get a uniform light-sheet across the FOV.

Cleared mouse embryo labeled with methylene blue (cyan) and showing autofluorescence (magenta) scanned with the LCS SPIM. Sample processed by Montserrat Coll Lladó, EMBL Barcelona.

The LuxBundle processing software allows stitching and processing data from large cleared samples.

For more information, check out the LCS SPIM website.

Detection objective

Eff. MagnificationFOV (µm)Pixel size (nm)Optical resolution
2.2x
59902925
Olympus 4x 0.28 NA air4.4x299514631.1µm
6.7x1997975
8.9x1497731

Available lasers

Wavelength [nm]
405
488
515
561
642
685

Available filters

Filters
BP 418-462
LP 498
BP 500-530
LP 530
BP 526-564
LP 572
BP 580-627
LP 656
BP 655-704
LP 696

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