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Mesoscopic Imaging Facility

NanoLive CX-A

The CX-A is an holotomography microscope designed to perform automated imaging of samples in 96-wells plates or 35mm Petri dishes. It allows to measure the refractive index of samples with sub-cellular resolution.

Thanks to the label-free nature of the technology and the stage-top incubation system, the CX-A is particularly suited for long-term live cell imaging.

24-hours time lapse movie of mouse ESCs (acquired by Kristina Stapornwongkul, field of view: ~100 micrometers)

Pixel intensities are a quantitative measure and are proportional to the dry mass, therefore subcellular structures such as lipid droplets, mitochondria, nuclear vesicles can be resolved.

The CX-A is equipped with an LED and 3 filter sets to optionally perform widefield fluorescence microscopy in the Dapi, FitC and TritC/Cy5 channels.

The EVE Analytics software additionally provides an integrated image analysis and quantification tool that delivers analysis of cell morphology, composition and population dynamics.

For more information, check out the CX-A website.

Available Objective Lenses

BrandTypeMag.
(Range)
NAImmersionF.o.V. [μm]Usage
 60x0.8Air90 x 90Detection

Available illuminations

Imaging typeWavelength [nm]
Holotomography interference520
EpifluorescenceLED

Available filter sets

Dapi + FitC + TritC/Cy5

Resolution

Holotomography: x,y,z: 200 x 200 x 400 nm

Epifluorescence: x,y: ~ 400 nm

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