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Metabolomics Core Facility

This facility provides services for analysis of metabolites and lipids.

The facility is equipped with a high-resolution mass spectrometry system (HRMS) coupled with liquid chromatography (UHPLC) and direct infusion (NanoMate) for separation and detection of various classes of small molecules, metabolites and lipids.

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Major projects and accomplishments

  • Identification and detection of various classes of small molecules, metabolites, and lipids in various biological matrices
  • EMBL-MCF mass spectral library from over 700 metabolites & EMBL-MCF 2.0 (LC-MS/MS library using low-adsorption chromatopgraphy)
  • Lipidomics profiling platform for comprehensive analysis of lipid species from multiple lipid classes (e.g. glycerols (TGs, DGs), phospholipids, free fatty acids, etc)
  • Relative quantitation of metabolites and lipids using internal standards
  • Analysis of drugs and related metabolites

Services provided

We provide services for mass spectrometry-based analysis of small molecules, metabolites and lipids.

We perform targeted and untargeted measurements of various classes of molecules ranging from small metabolites to lipids in a variety of biological matrices.

Further services

  • Global untargeted metabolomics and lipidomics profiling
  • Targeted metabolomics profiling
  • Free fatty acid analysis
  • Metabolite and lipid identification using MS and MS/MS analysis
  • Drug and drug metabolite analysis
  • Consultation of experimental study design
  • Data processing incl. metabolite & lipid identification (level 1 confidence), visualization, and statistical analysis
  • Data archiving and sharing/publishing

Acknowledgment and Authorship

As per EMBL Internal Publication Policy (No. 63); if the facility services were used in work described in a publication, the facility must be acknowledged. For acknowledgment, the following statement must be added:

We acknowledge the support of the EMBL Metabolomics Core Facility (MCF) in the acquisition and analysis of liquid chromatography-mass spectrometry data.

In addition, if a staff member of the Metabolomics Core Facility contributes substantially to a published work, the staff member should be included as an author in the published work.

More information on this topic can be found here: Recommended guidelines & Acknowledging and citing core facilities

Contact

Metabolomics Core Facility
EMBL Heidelberg,
Meyerhofstraße 1,
69117 Heidelberg, Germany

Tel: +49 6221 387-8705

E-mail: mcf@embl.de

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