Living as well as chemically fixed specimens can be imaged. Specific structures such as membranes or cytoskeleton can be stained with different fluorescent dyes and observed simultaneously.
Scanning through a stack of optical sections allows 3D reconstruction of the specimen.
AI-guided acquisition allows for overview imaging with automated zoom-in or photomanipulation jobs on regions of interest.
Objectives
- Objective EC Plan-Neofluar 2.5x/0.085 M27 air (FWD=8.8 mm)
- Objective Plan-Apochromat 10x/0.45 M27 air (FWD=2.1 mm)
- Objective Plan-Apochromat 20x/0.8 M27 air (FWD=0.55 mm)
- Objective Plan-Apochromat 40x/1.3 Oil DIC M27 (FWD=0.13 mm)
- Objective Plan-APOCHROMAT 63x/1,4 Oil DIC (FWD=0.19 mm)
Laser:
- 405 nm – 5 mW
- 488 nm – 10 mW
- 561 nm – 10 mW
- 640 nm – 5 mW
Confocal detectors:
- 3 Gallium Arsenide Phosphid-PMT (GaAsP-PMT) for fluorescence
- standard PMT as transmission detector
WF-Filters:
- EX BP 385/30 for DAPI
- EX BP 469/38 for FITC
- EX BP 555/30 for TRITC
- EX BP 631/33 for Cy5
- QBS 405+493+575+653
- EM QBP 425/30+514/30+592/25+709/100
Light Source HXP Lamp 120C (widefield)
Mercury short-arc reflector lamp 120 W. Ideal light source for fluorescence applications in microscopy. Integrated electronic shutter.
Camera: (widefield)
Camera Axiocam 503 mono
Number of Pixels: 1936 (H) x 1460 (V) = 2.8 Megapixels Pixel size: 4.54 μm x 4.54 μm Chip size: 8.8 mm x 6.6 mm, equivalent to 2/3″ (11 mm diagonal) Spectral range: With protection glass app. 400 nm to 1000 nm Max. Full Well Capacity: Approx. 15.000 e
Stage STEP Set for LSM:
- Scanning Stage 130×100 STEP with stepper motor (2 mm spindle pitch) incl. Stepper controller SMC 2009, Joystick XY, fixed motor cable with exit on stage left back side and universal mounting frame K (160 mm x 110 mm), also suitable for Z-Piezo
- travel range: 130 mm x 100 mm (adjustable)
- max. speed: 50 mm/s – resolution: 0.1 μm
- reproducibility: +/- 1 μm
- absolute accuracy: +/- 5 μm
Stage attachment Z PIEZO WSB 500 (D)
- travel range in Z: 500 μm
- max speed: 5 mm/sec
- resolution: 1 nm
- reproducibility: +/- 5 nm
