This facility provides a full proteomics infrastructure for the identification and characterisation of proteins.
Do’s and Don’t’s
Do
Fill out the request form and discuss your question before sending samples.
Contact the Facility at any time to discuss sample preparation / processing / shipping.
Provide us with as much information about your sample / protein as you can.
Use clean containers to stain and store gels, and use them only for this purpose.
Use a lid for the container mentioned above.
Wear gloves at all times during sample handling.
Cut gel bands on a clean bench and a clean glass plate using a clean (=new) scalpel knife,use 70% ethanol for cleaning and lint-free towels (like from Kimberly-Clark Kimtech science).
Send the protein sequence (FASTA format) when you have any tags attached to the original sequence (e.g. his tag, Flag, MBP, GST, etc.)
Acknowledge the Facility in your publications (please), if analyses / services have contributed to your work.
Use safe-lock tubes when sending samples to avoid opening of tubes during transport
If you send samples on dry ice, please snap-freeze them before in liquid nitrogen or take them directly from your freezer to avoid opening of tubes
Please spin samples shortly before freezing that the liquid is on the bottom of the tube
Don’t
Don’t ship samples over the weekend
Don’t incubate gels in the microwave to expedite coomassie staining
Don’t use overhead transparencies to scan gels
Don’t use detergents and/or >5% glycerol for MS experiments of intact proteins
Don’t send samples without discussing with the PCF staff