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Small Angle X-Ray Scattering

Sample Environments

SCOPE

At P12, users can conduct a variety of SAXS experiments, including automated BioSAXS for high-throughput data collection, time-resolved SAXS (TR-SAXS) to capture dynamic structural changes in biological macromolecules, and custom experiments requiring specialized conditions.

Automated BioSAXS allows on-site or mail-in experiments, while TR-SAXS and custom setups require designated proposals and staff collaboration for planning.

Automated BioSAXS

  • Automated BioSAXS at P12 supports high-throughput experiments, including data collection and downstream processing.
  • Experiments can be conducted on-site or through mail-in access, with beamline staff performing the work.
  • Three modules can run simultaneously during experiments:
    • Batch SAXS (static),
    • SEC-SAXS
    • AF4-SAXS (requires a special request).

Time Resolved SAXS

  • The Time-Resolved BioSAXS environment enables studying rapid structural changes in biological macromolecules under native-like conditions
  • Currently, we offer two modes:
    • Stopped-Flow Mixing (SFM-SAXS)
    • Laser-Triggered (Laser-SAXS)
  • Access has to be requested with the project proposal to have a designated beamtime allocated.
  • Prior discussion with beamline staff is strongly encouraged.
  • Pre-existing SAXS data in equilibrium is often required, or users may need to perform automated SAXS first.

Special Environments

  • Other Sample Environments at P12 support custom experiments using advanced SAXS techniques or user-specific setups.
  • This mode is ideal for experiments requiring unique conditions or equipment beyond standard configurations.
  • Users are encouraged to collaborate with beamline staff to plan and optimize their experiments.
  • Access to these sample environments is granted separately, similar to TR-SAXS, and requires staff input for experimental planning.

Automated SAXS

TR-SAXS

Special SAXS

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