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Small Angle X-Ray Scattering

Parameters

Synchrotron sourceStandard Petra U29 undulator
MonochromatorOxford double crystal monochromator, crystal Si (111) or Double Multilayer monochromator (DMM)
MirrorKB mirrors, Rh (-coated) and SiO3 (uncoated)
Control systemBeckhoff Twincat, TINE
Wavelength0.06 – 0.3 nm (DCM), 0.08 -0.17 (DMM)
Focal spot0.2 mm x 0.12 mm
Flux1013 ph/s (DCM), 5*1014 (DMM)
Slits4*2 pairs: two sets of Cinel water cooled slits, defining slits: motorized highly polished tungsten blade, guard slits: Hybrid Scatterless slits (Xenocs)
Automated sample changer2 x 96 wells (crystallization plates)
4 x 8 samples (200 µl PCR stripes) + 12 buffers (1.5 ml Eppendorf tubes)
temperature controlled storage (5-40°C)
SEC-SAXSFPLC/HPLC – Agilent BioInert system with autosampler
On-line multi-angle laser light scattering (MALLS) and dynamic light scattering (Wyatt TREOS-QELS/T-Rex).
Exposure unitQuartz capillary, diameter 1 or 2 mm, automated filling and rinsing, temperature controlled (7-80°C). Filling volume: 30-40 µl for standard continuous flow operations (down to 5 µl for static measurements).
Alternative sample environmentsYZ- alignment stage for the positioning of user-made sample cell/holder.
Temperature controlled (0-100°C) capillary holder (Ø 1.5mm).
Stopped flow SFM-400 (Bio-Logic)
DetectorsPhoton counting Pilatus 6M (423.6 x 434.6mm2), Pilatus 2M pixel detector (253 x 288 mm2), Pilatus 1M-W detector (423.6 x 70 mm2), Eiger 4M detector (155.2 x 162.5 mm2, 750 Hz frame rate)
Sample-detector distanceTunable: 1.6 to 6.1 m
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