Eukaryotic Expression Facility

Two biosafety level 1 labs for protein expression in insect cells via notably the MultiBac-EMBL system:

• Introduction to baculovirus/insect cell expression
• Introduction to biGBac and MultiBac systems for multiprotein complex production
• Providing transfer vectors and protocols for multigene expression plasmid generation
• Providing E. coli cells for composite bacmid generation
• Providing insect cells for virus generation and amplification
• Viral performance and protein production monitoring
• Small, medium, and large-scale expression of proteins and protein

Two biosafety level 2 labs for the growth of mammalian cells, either adherent or in suspension:

• Introduction to mammalian cell culture
• Introduction to recombinant protein expression in mammalian cell
• Design of constructs for the generation of stable cell line
• Support in stable cell line establishment, notably using CRISPR/Cas9 and PiggyBac transposons systems
• Providing standard vectors for stable cell line generation
• Providing standard vectors for constitutive or conditional recombinant expression
• Providing reagents and protocols for large cargo delivery using BacMAM

In addition to all equipment for the culture of eukaryotic cells, the platform also offers access to Light and Confocal Microscopes.

Researchers are kindly requested to acknowledge the Eukaryotic Expression Facility (EEF) at EMBL Grenoble in all publications arising from work involving insect or mammalian cell systems conducted at the facility. Please include the following statement in the acknowledgements section of your manuscript prior to submission:

“We acknowledge Martin Pelosse for support in using the Eukaryotic Expression Facility at EMBL Grenoble.”

For collaborative work, platform staff must co-sign papers.”