23 March - 28 March 2025
Measuring translational dynamics by ribosome profiling
Pasha Baranov, Jan Medenbach, Sebastian Leidel, Elisabeth Zielonka
Quantitative assessment of mRNA translation is critical to understanding gene expression in many biological settings In this practical course we will summarise the state of the art in the theoretical sessions combined with hands on experiments and computational analyses Participants will learn a robust protocol and how to adapt it to different experimental systems and also learn the key steps of computational quality control... OverviewSpeakers, trainers and staffProgrammePractical informationMedia kitSponsors Overview Course overview Regulation of translation is key in the control of gene expression, playing a critical role in many biological and pathophysiological settings. In the last decade, methodological advances have provided comprehensive insights into cellular transcriptomes and proteomes, however, the systems-wide analysis of translation remained an unsolved experimental challenge. The advent of ‘ribosome-profiling’ provided the methodological basis for comprehensive transcriptome-wide analyses of translation at sub-codon resolution by the precise and high-throughput determination of ribosomal positions on mRNA. Its application in a variety of organisms yielded novel insights into protein synthesis and its dynamic regulation, significantly advancing our understanding of the post-transcriptional...
EMBO Practical Course
EMBL Heidelberg
Closed